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Comparison of Serum Valproic Acid Determination Through Gas and High Performance Liquid Chromatography Methods

Ali Nazeri 1 , Maryam Jalali 2 , Mehri Aliasgharpour 1 , * and Farideh Khosravie 2
Authors Information
1 Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, IR Iran
2 Noor Pathobiology Laboratory, Tehran, IR Iran
Article information
  • Health Scope: February 24, 2014, 3 (1); e12085
  • Published Online: February 17, 2014
  • Article Type: Research Article
  • Received: May 11, 2013
  • Revised: June 24, 2013
  • Accepted: August 2, 2013
  • DOI: 10.17795/jhealthscope-12085

To Cite: Nazeri A, Jalali M, Aliasgharpour M, Khosravie F. Comparison of Serum Valproic Acid Determination Through Gas and High Performance Liquid Chromatography Methods, Health Scope. 2014 ; 3(1):e12085. doi: 10.17795/jhealthscope-12085.

Copyright © 2014, Health Promotion Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Bowden CL, Singh V. Valproate in bipolar disorder: 2000 onwards. Acta Psychiatr Scand Suppl. 2005; (426): 13-20[DOI][PubMed]
  • 2. Perucca E. Pharmacological and therapeutic properties of valproate: a summary after 35 years of clinical experience. CNS Drugs. 2002; 16(10): 695-714[PubMed]
  • 3. Haddad PM, Das A, Ashfaq M, Wieck A. A review of valproate in psychiatric practice. Expert Opin Drug Metab Toxicol. 2009; 5(5): 539-51[DOI][PubMed]
  • 4. DeVane CL. Pharmacokinetics, drug interactions, and tolerability of valproate. Psychopharmacol Bull. 2003; 37 Suppl 2: 25-42[PubMed]
  • 5. Bentué-Ferrer D, Tribut O, Verdier MC. [Therapeutic drug monitoring of valproate]. Therapie. 2009; 65(3): 233-240
  • 6. Kazuko K, Mikio N, Ryota N, Satoshi K. Direct method for determination of valproic acid in serum by HPLC. Analytical Sci. 1985; 1: 385-7
  • 7. Wohler AS, Poklis A. A simple, rapid gas-liquid chromatographic procedure for the determination of valproic acid in serum. J Anal Toxicol. 1997; 21(4): 306-9[PubMed]
  • 8. Berry DJ, Clarke LA. Determination of valproic acid (dipropylacetic acid) in plasma by gas-liquid chromatography. J Chromatogr. 1978; 156(2): 301-7[PubMed]
  • 9. Hershey AE, Patton JR, Dudley KH. Gas chromatographic method for the determination of valproic acid in human plasma. Ther Drug Monit. 1979; 1(2): 217-41[PubMed]
  • 10. Leroux M, Budnik D, Hall K, Irvine-Meek J, Otten N, Seshia S. Comparison of gas-liquid chromatography and EMIT assay for serum valproic acid. Clin Biochem. 1981; 14(2): 87-90[PubMed]
  • 11. Irshaid YM, Hamdi AA, Al Homrany M. Evaluation of therapeutic drug monitoring of antiepileptic drugs. Int J Clin Pharmacol Ther. 2003; 41(3): 126-31[PubMed]
  • 12. Bowers LD, Armbruster DA, Cairns T, Cody MJT, Fitzgerald R, Goldberger BA, et al. Gas Chromatography/Mass Spectrometry (GC/MS) Confirmation of Drugs; Approved Guideline. 2002;
  • 13. Dowling S. Clarke's analysis of drugs and poisons: in Pharmaceuticals, body fluids and postmortem material. 2004;
  • 14. Kebriaeizadeh A, Valaie M, Zarghi A. Determination of valproic acid in human plasma by gas chromatography. Pharm Pharmacol Commun. 1998; 4(11): 525-7
  • 15. Hara S, Kamura M, Inoue K, Fukuzawa M, Ono N, Kuroda T. Determination of valproic acid in human serum by high-performance liquid chromatography with fluorescence detection. Biol Pharm Bull. 1999; 22(9): 975-7[PubMed]
  • 16. Liu H, Forman LJ, Montoya J, Eggers C, Barham C, Delgado M. Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection. J Chromatogr B: Biomed Sci Appl. 1992; 576(1): 163-9
  • 17. Elyas AA, Goldberg VD, Ratnaraj N, Lascelles PT. Valproic acid estimation by enzyme immunoassay. Ann Clin Biochem. 1980; 17(6): 307-10[PubMed]
  • 18. Godolphin W, Trepanier J, Farrell K. Serum and plasma for total and free anticonvulsant drug analyses: effects on EMIT assays and ultrafiltration devices. Ther Drug Monit. 1983; 5(3): 319-23[PubMed]
  • 19. Kumps AH. Therapeutic drug monitoring: a comprehensive and critical review of analytical methods for anticonvulsive drugs. J Neurol. 1982; 228(1): 1-16[PubMed]
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